Cannabis-induced cytotoxicity in leukemic cell lines: the role of the cannabinoid receptors and the MAPK pathway.

Delta9-Tetrahydrocannabinol (THC) is the active metabolite of cannabis. THC causes cell death in vitro through the activation of complex signal transduction pathways. However, the role that the cannabinoid 1 and 2 receptors (CB1-R and CB2-R) play in this process is less clear. We therefore investigated the role of the CB-Rs in mediating apoptosis in 3 leukemic cell lines and performed microarray and immunoblot analyses to establish further the mechanism of cell death. We developed a novel flow cytometric technique of measuring the expression of functional receptors and used combinations of selective CB1-R and CB2-R antagonists and agonists to determine their individual roles in this process. We have shown that THC is a potent inducer of apoptosis, even at 1 x IC(50) (inhibitory concentration 50%) concentrations and as early as 6 hours after exposure to the drug. These effects were seen in leukemic cell lines (CEM, HEL-92, and HL60) as well as in peripheral blood mononuclear cells. Additionally, THC did not appear to act synergistically with cytotoxic agents such as cisplatin. One of the most intriguing findings was that THC-induced cell death was preceded by significant changes in the expression of genes involved in the mitogen-activated protein kinase (MAPK) signal transduction pathways. Both apoptosis and gene expression changes were altered independent of p53 and the CB-Rs.

Artículo completo: https://www.ncbi.nlm.nih.gov/pubmed/15454482

ARTICULOS

cannabis de esperanza

Naciones Unidas trabaja en facilitar “la investigación y comercio de cannabis mundial” (FAAAT Carta Abierta)

La primera publicidad educativa cannábica aprobada en Londres (TRADUCCIÓN)

Cannabis medicinal: reglamento del Minsa aún margina a pacientes (LA ENTREVISTA)

Perú: Convenio Dará Cannabis Medicinal Sin Costo a Pacientes de Bajos Recursos

Encuesta Federal Identifica Notorio Decrecimiento en El Uso de Marihuana en Gente Joven (TRADUCCIÓN)

LA ONU Y LA OMS CIERRAN 60 AÑOS DE PARÉNTESIS OSCURANTISTA (PARTE 1)

VIDEOS

Cannabis Medicinal y Fibromialgia: El Camino de Libertad

El científico (The Scientist – subtitulos en español)

Primera Charla Gratuita Cannabis Medicinal, Dr. Dave Hepburn MD

Cannabis Medicinal y Fibromialgia: El Camino de Libertad

El científico (The Scientist – subtitulos en español)

Primera Charla Gratuita Cannabis Medicinal, Dr. Dave Hepburn MD

Open chat